FIG. 1.

SDS-PAGE of Cry1Ac toxin and domain III mutants after trypsin digestion. Solubilized protoxin was digested with 2% trypsin (wt/wt) for 2 h at 37°C. An additional dose of 1% trypsin was freshly added, and the mixture was further incubated for 1 h. The purity of activated toxins was examined by SDS-10% PAGE. Lane 1, molecular marker; lane 2, Cry1Ac; lane 3, ⁵⁰³SS⁵⁰⁴; lane 4, ⁵⁰⁶NNI⁵⁰⁸; lane 5, ⁵⁰⁹QNR⁵¹¹; lane 6, ⁵²²ST⁵²³; lane 7, ⁵²⁴ST⁵²⁵; lane 8, ⁵⁰⁹Q-A; lane 9, ⁵¹⁰N-A; lane 10, ⁵¹¹R-A; lane 11, ⁵¹³Y-A.