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Binding of Cry1Ac domain III mutant toxins to M. sexta BBMV. 125I-labeled Cry1Ac (2 nM) was incubated with 10 μg of BBMV in the presence of increasing concentrations of unlabeled Cry1Ac, 509QNR511-AAA, 509Q-A, 510N-A, 511R-A, and 513Y-A mutant toxins. Binding is expressed as percentage of the amount bound upon incubation with labeled toxin alone.
