TABLE 1.

Biological activity and binding parameters of Cry1Ac and mutant toxins to L. dispar

Toxin	LC50 (ng/cm2)a	Relative toxicityb	Kcom (nM)c
Cry1Ac	7.98 (5.04–11.02)	1	3.7 ± 0.8
503SS504	7.35 (4.67–10.58)	1.08	4.2 ± 0.9
506NNI508	8.15 (5.68–11.26)	0.97	4.5 ± 1.1
509QNR511	26.94 (23.48–30.63)	0.30	38.5 ± 5.3
522ST523	8.93 (6.74–12.15)	0.89	4.6 ± 0.9
524ST525	8.53 (5.83–11.64)	0.94	4.7 ± 1.1
509Q	17.96 (14.46–21.56)	0.43	18.5 ± 3.5
510N	9.23 (7.21–12.54)	0.86	3.5 ± 0.9
511R	19.84 (16.34–23.14)	0.40	32.5 ± 5.2
513Y	22.81 (18.31–27.21)	0.35	35.5 ± 5.9

^aNinety-five percent confidence limits are given in parentheses. 

^bRelative toxicity yielded by wild-type toxin LC₅₀/mutant toxin LC₅₀. 

^cDissociation constant calculated from homologous competition binding assays.