TABLE 2.
Biological activity and binding parameters of Cry1Ac and mutant toxins to M. sexta
| Toxin | LC50 (ng/cm2)a | Relative toxicityb | Kcom (nM)c |
|---|---|---|---|
| Cry1Ac | 4.68 (3.16–6.23) | 1 | 3.9 ± 1.2 |
| 503SS504 | 4.75 (3.12–6.53) | 0.99 | 4.3 ± 1.4 |
| 506NNI508 | 4.81 (3.33–6.65) | 0.97 | 4.8 ± 1.3 |
| 509QNR511 | 8.61 (6.93–10.34) | 0.54 | 38.2 ± 5.3 |
| 522ST523 | 4.56 (3.04–6.21) | 1.03 | 4.1 ± 1.4 |
| 524ST525 | 5.13 (3.65–6.84) | 0.91 | 4.5 ± 1.6 |
| 509Q | 7.53 (5.72–9.23) | 0.62 | 17.3 ± 3.7 |
| 510N | 4.46 (3.02–6.11) | 1.05 | 4.7 ± 1.7 |
| 511R | 8.13 (6.40–9.94) | 0.58 | 31.8 ± 6.3 |
| 513Y | 9.79 (8.19–11.52) | 0.48 | 22.5 ± 4.5 |
a
Ninety-five percent confidence limits are given in parentheses.
b
Relative toxicity yielded by wild-type toxin LC50/mutant toxin LC50.
c
Dissociation constant calculated from homologous competition binding assays.