TABLE 3.

Biological activity and binding parameters of Cry1Ac and mutant toxins to H. virescens

Toxin	LC50 (ng/cm2)a	Relative toxicityb	Kcom (nM)c
Cry1Ac	2.83 (1.75–4.24)	1	1.1 ± 0.3
503SS504	1.40 (0.80–2.24)	2	1.6 ± 0.4
506NNI508	0.76 (0.48–1.14)	3.7	1.5 ± 0.3
509QNR511	11.03 (6.99–16.47)	0.26	24.3 ± 4.2
522ST523	1.21 (0.76–1.84)	2.3	1.8 ± 0.5
524ST525	1.13 (0.71–1.72)	2.5	1.6 ± 0.5
509Q	1.98 (1.26–3.06)	1.4	8.6 ± 2.2
510N	3.01 (1.77–5.17)	0.94	1.5 ± 0.5
511R	8.99 (5.82–14.21)	0.31	17.6 ± 3.4
513Y	1.60 (0.395–4.55)	1.8	12.3 ± 3.2

^aNinety-five percent confidence limits are given in parentheses. 

^bRelative toxicity yielded by wild-type toxin LC₅₀/mutant toxin LC₅₀. 

^cDissociation constant calculated from homologous competition binding assays.