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. 2022 May 19;13:818173. doi: 10.3389/fimmu.2022.818173

Figure 3.

Figure 3

Endogenous and exogenous estrogen promoted psoriasis. Comparison of psoriasis scores (PASI): (A) Ovariectomized (OVX) and sham operated mice with or without mannan application (n = 10/group); (B) single 17-β-estradiol or miglyol alone treated mice without any mannan application (n = 10/group); (C) 17-β-estradiol or miglyol alone injected mice with mannan application (n = 10/group); (D) ER-β antagonist (PHTPP) or corn oil alone treated mice (n = 5/group), (E) ER-β agonist (DPN) or corn oil alone treated mice (n = 5/group). Representative pictures of H&E-stained skin samples at peak of psoriasis from (F) OVX and sham operated mice, (G) 17-β-estradiol treated OVX mice, without and with mannan stimulation, and (H) after PHTPP and DPN treatment. (I, J) Epidermis thickness (n = 5/group) and histological scores of skin sections (n = 3/group) at Day 5 were calculated from Figures (F–H). Scale bar: 200 µm. Each experiment was repeated twice. Statistical analyses were performed using an unpaired t test and n indicates number of mice. The data represent mean ± SEM. Man, mannan; PO, PBS + OVX; MO, Man + OVX; PS, PBS + sham; MS, Man + Sham; Mig, miglyol; E2, 17-β-estradiol; Oil (P), E2 + Corn Oil + Man; PHTPP, E2 + PHTPP + Man; Oil (D), Corn Oil + Man; DPN, DPN + Man. The data represent mean ± SEM. ns, not significant. *p < 0.05; **p < 0.01. ***p < 0.001.