Figure 6.

Estrogen increased ER-β expression in CD11c⁺ cells in the inflamed skin. (A) Immunofluorescence staining of ER-β (red) and CD11c (green) double positive cells in male and female mice under normal and inflammatory conditions (n = 3/group); effect of (B) endogenous estrogen, (C) 17-β-estradiol, (D) ER-β antagonist or agonist on the expression of ER-β and CD11c double positive cells (n = 3/group). (E–H) Quantification of CD11c⁺ER-β⁺ positive cells by immunofluorescence (n = 3/group). Scale bar: 200 µm. Nuclei were counterstained with DAPI (blue). Representative pictures are shown. Statistical analyses were performed using an unpaired t test. Man, Mannan; NM, Naive Male; MM, Man + Male; NF, Naive Female; MF, Man + Female; PO, PBS + OVX; MO, Man + OVX; PS, PBS + Sham; MS, Man + Sham; Mig, miglyol; E2, 17-β-estradiol; Oil (P), E2 + Corn oil + Man; PHTPP, E2 + PHTPP +Man; Oil (D), Corn oil + DPN; DPN, DPN + Man. n, number of mice. The data represent mean ± SEM. ns, not significant. *p < 0.05; **p < 0.01. ***p < 0.001.