Figure 7.

Estrogen enhanced mannan-induced skin inflammation was promoted by γδ T cells. (A) Percentage of CD45⁺γδ T⁺ cells in the dermis. (B) Representative pictures of CD45⁺γδ T⁺ cell expression after 17-β-estradiol treatment in MISI (n = 5/group). (C–F) γδ T⁺ cells in the draining lymph nodes from female and male mice, OVX and sham operated mice, 17-β-estradiol, miglyol, PHTPP or DPN treated mice with and without mannan application (n = 5/group). Each experiment was repeated twice. Man, mannan; NM, Naive Male; MM, Man + Male; NF, Naive Female; MF, Man + Female; PO, PBS + OVX; MO, Man + OVX; PS, PBS + Sham; MS, Man + Sham; Mig, miglyol; E2, 17-β-estradiol; Oil (P), E2 + Corn oil + Man; PHTPP, E2 + PHTPP + Man; Oil (D), Corn oil + Man; DPN, DPN + Man. Statistical analyses were performed using an unpaired t test and n indicates number of mice. The data represent mean ± SEM. ns, not significant. *p < 0.05; **p < 0.01. ***p < 0.001.