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. 2022 Jun 1;12:9116. doi: 10.1038/s41598-022-13231-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Determination of Rankl and Opg gene expression in miRNA mimic-transfected chondrocytes and in cultured chondrocytes from Mirc24 cluster-deficient mice. The gene expression of Rankl and Opg was analyzed by semi-quantitative PCR (a) in control (ctrl), miR-322 (322) or miR-503 (503) mimic-transfected primary epiphyseal chondrocytes (PECs) 2 days post transfection and (b) in PECs isolated from newborn male Col2a1-Cre (Cre) and Col2a1-Cre-Mirc24^tm1M/Y (hKO) mice and cultured for 2 days. The control (H₂O) contains no genomic DNA and Actb served as loading control. Rankl or Opg to Actb ratio was determined by ImageJ and individual and mean values and standard deviations are shown (graph). The size of NEB Quick-Load Purple 1 kb Plus DNA Ladder bands is shown. The figure was created using Adobe Illustrator CS6 (Version 16.0.3, https://adobe.com/products/illustrator).