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. 2021 Aug 23;43(6):1544–1555. doi: 10.1038/s41401-021-00757-7

Fig. 6. Osthole suppresses ITGα3 and ITGβ5 downstream FAK/Src/Rac1 signaling.

Fig. 6

MDA-MB-231 and MDA-MB-231BO cells with shITGα3 or shITGβ5, and MCF-7 cells with ITGα3-OE or ITGβ5-OE were treated with 10 μg/mL of osthole for 48 h, respectively. a The mRNA expression of FAK, Src, paxillin (PXN), and Rac1 was evaluated by real-time PCR. *P < 0.05, **P < 0.01 vs. the shCtrl or the vector group; #P < 0.05, ##P < 0.01 vs. the shITGα3 or the ITGα3-OE group; $P < 0.05, $$P < 0.01 vs. the shITGβ5 or the ITGβ5-OE group. b–d The protein expression of FAK, p-FAK, Src, PXN, p-PXN, and Rac1 in MDA-MB-231 (b), MDA-MB-231BO (c), and MCF-7 (d) cells was analyzed by immunofluorescence assay. Scale bar = 20 μm. *P < 0.05, **P < 0.01 vs. the shCtrl or the vector group; ##P < 0.01 vs. the ITGα3-OE group; $$P < 0.01 vs. the ITGβ5-OE group. shCtrl: control vectors; MFI: mean fluorescence intensity.