Skip to main content
. 2021 Aug 23;43(6):1544–1555. doi: 10.1038/s41401-021-00757-7

Table 1.

Sequences of forward and reverse primers used in real-time PCR analysis.

Genes Sequences
β-actin

Forward: 5’-TGACGTGGACATCCGCAAAG-3’

Reverse: 5’-CTGGAAGGTGGACAGCGAGG-3’

ITGαv

Forward: 5’-AACCCAAGCTGGAAGTTTCTG-3’

Reverse: 5’-GCAGTGGAATGGAAACGATG-3’

ITGα2

Forward: 5’-GGACTTTCGCATCATCAACG-3’

Reverse: 5’-GGTACTTCGGCTTTCTCATCAG-3’

ITGα3

Forward: 5’-ACCCCAACTACAGGCGAAAC-3’

Reverse: 5’-AGAAGAAGCCGTGGAAGACAG-3’

ITGβ5

Forward: 5’-ATCCCTTGCCTTGCTTGG-3’

Reverse: 5’-CACCGTTGTTCCAGGTATCAG-3’

FAK

Forward: 5’-CAGCTCAGCACAATCCTGGAG-3’

Reverse: 5’-TTCGCTGGACCTCGGACTG-3’

Src

Forward: 5’-CTTTGGCGAGGTGTGGATG-3’

Reverse: 5’-GTAAATGGGCTCCTCTGAAACC-3’

Rac1

Forward: 5’-GGCACCACTGTCCCAACAC-3’

Reverse: 5’-GCACTCCAGGTATTTTACAGCAC-3’

Paxillin

Forward: 5’-CTTTGGTCCCGAAGGGTTC-3’

Reverse: 5’-AGGATGCCACAGCGTGTTG-3’

HSP70

Forward: 5’-ACTGTGGACCTGCCAATCG-3’

Reverse: 5’-GCATCATTCCGCTCCTTCTC-3’

Moesin

Forward: 5’-TTAAGTTCCGTGCCAAGTTCTAC-3’

Reverse: 5’-AGGCGGGCAGTAAATATCATC-3’

S100A

Forward: 5’-CCCTCATCAACGTGTTCCAC-3’

Reverse: 5’-CATCACCTTGTCCACAGCATC-3’