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. 2021 Sep 14;43(6):1395–1407. doi: 10.1038/s41401-021-00763-9

Fig. 6. Phosphoinositide 3-kinase (PI3K)/Akt is involved in AA-induced glycophagy.

Fig. 6

a, b Representative Western blots (a) and quantitative analysis (b) of Akt and glycogen synthase kinase 3 beta (GSK3β) phosphorylation in isolated cardiomyocyte lysates, n = 4 independent experiments. c Co-immunoprecipitation of STBD1 and GABARAPL1 in isolated cardiomyocytes treated with AA (10 μmol/L; before OGD), PI3K inhibitor LY294002 (LY; 20 μmol/L), or Akt inhibitor GSK690693 (GSK; 10 μmol/L) and then subjected to OGD. The expression levels of Akt and GSK3β in cell lysates were also verified. d ECAR analysis was carried out to determine the glycolytic activity in isolated cardiomyocytes, n = 6 independent experiments. **P < 0.01 vs. OGD group, ##P < 0.01 vs. OGD + DMSO group. &&P < 0.01 vs. OGD + AA 10 + DMSO group. Data are presented as the mean ± SEM.