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. 2022 Jun 2;11:154. doi: 10.1038/s41377-022-00815-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Setup of TIDT-NSA system based on a commercial microscope equipped with a multi-annular programmable LED source at the front-end of illumination and a scanning drive mechanism at the back-end of acquisition. b A photo of our TIDT-NSA hardware system. c Flowchart of data processing in TIDT-NSA for 3D RI reconstruction on the example of unstained MCF-7 cells.