Skip to main content
. 2021 Aug 30;43(6):1556–1567. doi: 10.1038/s41401-021-00748-8

Fig. 3. MCP reduced the ROS level in TAM not only through galectin-3 inhibition, but also through its reducibility.

Fig. 3

The protein (a) and mRNA (b) expression levels of galectin-3 in TAM treated with MCP for 24 h were detected by Western blotting and RT-PCR methods. c Effect of siGal-3 on the ROS level was detected by flow cytometry method. d Effect of siGal-3 on the glucose uptake of TAM treated with MCP in hypoxia for 24 h was detected by glucose assay kit. e Redox capacity of MCP was detected by antioxidant assay. f Effects of MCP and siGal-3 on the phosphorylation of Akt in TAM were detected by Western blotting method. g Immunofluorescence images of GLUT-1 expression in hypoxia TAM treated with MCP or siGal-3 (bar = 20 μm). All the experiments have been repeated three times. *P < 0.05, **P < 0.01, vs. respective normaxia control group, #P < 0.05, ##P < 0.01, vs. respective hypoxia group.