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. 2022 May 19;16:820396. doi: 10.3389/fncel.2022.820396

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Copyright © 2022 Ning, Zheng, Xie, Gao, Xu, Xu, Wang, Peng, Jiang, Ge and Zhong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Optimal expansion of induced MGCs (iMGCs) from hiPSC-derived ROs. (A) Schematic diagram of expansion of iMGCs from hiPSC-derived ROs. (B) Bright-field images showed the distinguished morphological features of the passage 0 (P0) cells isolated from D90-ROs (D90-P0) and D150-ROs (D150-P0). (C) The cell doubling time showed different expansion capability of cells isolated from D60-, D90-, D120-, and D150-ROs. (D) Bright-field images showed no obvious morphological difference in P1, P3, and P5 cells expanded from D90- and D150-ROs, all appearing a spindle-like shape. ROs, retinal organoids; NR, neural retina. Scale bars = 200 μm (B) and 100 μm (D).