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. 2022 May 19;16:820396. doi: 10.3389/fncel.2022.820396

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Copyright © 2022 Ning, Zheng, Xie, Gao, Xu, Xu, Wang, Peng, Jiang, Ge and Zhong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression of cell-specific markers in passaged iMGCs from early- and late- stage ROs. (A) Immunofluorescence staining showed the expression of markers SOX9, vimentin, nestin, VSX2, PAX6, CRX, and GS in the passaged cells isolated from D90- and D150-ROs. (B) Percentage of positive cells in (A). Cell number > 100, *p < 0.05. (C) Schematic diagram of molecular characteristics of iMGCs isolated from the late-stage ROs. ROs, retinal organoids; ns, no significance; Scale bars = 50 μm (A).