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. 2022 May 19;16:820396. doi: 10.3389/fncel.2022.820396

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Copyright © 2022 Ning, Zheng, Xie, Gao, Xu, Xu, Wang, Peng, Jiang, Ge and Zhong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ultrastructural and electrophysiological characteristics of the passaged iMGCs. (A) SEM images showed the iMGCs possessed a typical spindle-like morphology with many microvillus. (B) TEM images showed the iMGCs with abundant mitochondria (M), endoplasmic reticulum (ER), Golgi apparatus (G), glycogen (Gly), microtubules (MT), intermediate filaments (IF) and actins (A) in the cytoplasm. (C) Both P1 and P5 iMGCs depolarized after application of L-glutamate in current-clamp recordings. (D) P1 and P5 iMGCs presented no significant difference in resting membrane potential and the magnitude of depolarization. SEM, scanning electron microscope; TEM, transmission electron microscope; P1 and P5, passage 1 and 5; ns, no significance.