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. 2022 May 19;16:820396. doi: 10.3389/fncel.2022.820396

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Copyright © 2022 Ning, Zheng, Xie, Gao, Xu, Xu, Wang, Peng, Jiang, Ge and Zhong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of cell survival of the passaged iMGCs after subretinal transplantation into NOD/SCID mice. (A) Schematic overview of the subretinal transplantation process of the iMGCs. (B,C) Fundus photography (B) and optical coherence tomography (OCT) (C) showed the successful delivery of the iMGCs into subreinal space (SRS) in NOD/SCID mice 3 days after transplantation (DAT). *The grafted cells. (D,E) Immunofluorescence staining showed the grafted iMGCs (D3, P2 iMGCs from D120 BC1-ROs) were positive for HNA and SOX9, but negative for VSX2, PAX6, and CRX at 3 DAT, and gradually disappeared from 7 to 21 DAT. INL, inner nuclear layer; ONL, outer nuclear layer. Scale bars = 50 μm (D,E).