Figure 9.

Deletion of CKS2 inhibits the proliferation and invasive abilities of glioma cell lines. (A) On the basis of the qRT-PCR analysis, CKS2 expression was particularly upregulated in glioma tissues (n = 34) compared to normal brain tissues (n = 10). (B) The association between CKS2 expression and histological grade (n = 34). (C, D)  In U251 and U87 cells, the expression of CKS2 was downregulated in the siRNA group. (E) The expression of CKS2 in various glioma cell lines. (F, G) MTS assay to detect U251 transfected with si-CKS2 and the proliferation inhibition of U87 cells. (H, I) Colony formation and transwell assays showed that CKS2 downregulation greatly hindered the proliferation and invasion of U251 and U87 cell lines compared with the control group. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.