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. 2022 May 23;12(4):439–443. doi: 10.1016/j.jobcr.2022.05.008

Table 2.

Changes in the oral karitinocytes exposed to shisha extract (In-vitro studies).

Author (year) In vitro testing Findings
S.Patil et al. 201927 Oral keratinocytes chronically exposed to shisha and their multiomic analysis In cells treated with shisha extract, two hundred forty-seven genes expressed differently. Changes in the activate MAPK 1 pathway and the interferon signaling pathway were demonstrated. RNA-Seq dataset noted allele-specific expression of mutant LIMA1.
S.Patil et al. 201928 Examination of phosphoproteome of oral keratinocytes with chronic shisha exposure
  • 1

    Increased cell scattering phenomenon in OKF6/TERT1 cells.

  • 2

    Initiation of protein translation with proteins associated with mTORC1 and EIF4F complexes.

  • 3

    Downregulation of proteins involved in Type I interferon signaling.

  • 4

    It highligths global perturbations to the molecular milieu of oral keratinocytes.
S.Patil et al. 201929 Proteomic changes tested in Oral keratinocytes chronically exposed to Shisha
  • 1

    To cellular transformation and immune response dysregulation.

  • 2

    The results showed 5515 protein on proteomic analysis of oral keratinocytes of chronically shisha treated cells.

  • 3

    There was increased protein production involved with cell growth and reduced immune process. The interferon pathway was dysregulated
S.Patil et al. 201930 Oral keratinocytes chronically exposed to shisha and their secretome analysis
  • 1

    Shisha treated OKF6/TERT1- cells showed secretory potential of differentially secreted proteins.

  • 2

    Expression of AKR1C2, HSPH1, and MMP9 was validated in western blotting of shisha treated cells.

  • 3

    The secretome was in agreement with proteomic data.