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. 2022 Apr 28;28(7):1081–1092. doi: 10.1111/cns.13845

TABLE 1.

Primary antibodies used in IF and WB

Antibodies Source Catalog RRID Dilution Usage
GFAP Abcam ab33922 AB_732571 1:1000 IF
Nestin Abcam ab11306 AB_1640723 1:500 IF
DCX Abcam ab254133 N/A 1:500 IF
Ki67 Abcam ab16667 AB_302459 1:500 IF
DCX Abcam ab254133 N/A 1:1000 WB
BDNF Abcam ab205067 N/A 1:2000 WB
PI3KCA Abcam ab183957 N/A 1:1000 WB
Phospho‐Akt1/2/3 Abcam ab192623 AB_2269641 1:1000 WB
NT‐3 Abcam ab263864 AB_2884942 1:1000 WB
Akt3 CST #14982 AB_2716311 1:1000 WB
GAPDH CST #5174 AB_10622025 1:1000 WB
Ras CST #3339 AB_2269641 1:1000 WB
Phospho‐MEK1/2 CST #9154 AB_2138017 1:1000 WB
Phospho‐p44/42 MAK CST #4370 AB_2315112 1:1000 WB
Phospho‐PI3K p85/p55 Affinity AF3242 AB_2834668 1:1000 WB

Abbreviations: N/A, not available; CST, cell signaling technology; IF, immunofluorescence; WB, Western blotting.