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. 2022 Apr 10;28(7):1045–1058. doi: 10.1111/cns.13837

FIGURE 8.

FIGURE 8

Inhibiting ROS production with NAC partially attenuated PM2.5‐induced NLRP3 inflammasome activation and pyroptosis under ischemic conditions. (A) The production of ROS in control, OGD/R and OGD/R+PM2.5 groups (* p < 0.05 vs. control; #p < 0.05 vs. OGD/R; n = 3). (B) The production of ROS in OGD/R+Veh, OGD/R+PM2.5+Veh, OGD/R+NAC, and OGD/R+PM2.5+NAC groups. (C, D) The expressions of NLRP3, ASC, pro‐caspase‐1, Caspase‐1, GSDMD, and GSDMD‐N in four groups. (E, F) The production of inflammatory cytokines IL‐1β and IL‐18 in four groups. (G) Caspase‐1 activity was assessed in four groups (*p < 0.05, **p < 0.01, ***p < 0.001 vs. OGD/R+Veh; #p < 0.05, ##p < 0.01, ###p < 0.001 vs. OGD/R+PM2.5+NAC; n = 3). (H) The detection of DAPI and SYTOX green‐positive cells in the four groups. Scale bar = 20 μm