FIGURE 10.

HO-induced ROS generation in MLE-12 cells is attenuated by MitoTEMPO. MLE-12 cells were pretreated with vehicle or MitoTEMPO (10 µm) for 1 h and challenged with HO for 48 h. Mitochondrial superoxide production was assayed by MitoSOX red reagent (5 µm) (A) Representative images of MLE-12 cells stained with MitoSOX and (B) Quantified fluorescence intensity measured as arbitrary units showed an increase in MitoSOX staining with HO and decrease with MitoTEMPO treatment. n = 3. ****p < 0.0001.