FIGURE 11.

MitoTEMPO attenuates HO-induced apoptosis in MLE-12 cells. MLE-12 cells were pretreated with 10uM MitoTEMPO for 1 h followed by HO for 48 h. The cells were stained with Annexin V and 7-AAD and were subjected to flow cytometry. (A) Representative dot plots of cells after staining with Annexin V and 7-AAD (B) Quantification of the percentage of Annexin V- positive apoptotic cells which is expressed as fold-change showing increased apoptotic cells in HO which is significantly reduced with MitoTEMPO treatment. n = 4. ***p < 0.001, *p < 0.05.