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. 2022 May 19;13:880878. doi: 10.3389/fphar.2022.880878

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Copyright © 2022 Harijith, Basa, Ha, Thomas, Jafri, Fu, MacFarlane, Raffay, Natarajan and Sudhadevi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NOX4 expression is downregulated in the lungs and isolated AT2 cells from Nox4 ^−/− Spc-Cre mice. Nox ^+/- Spc-Cre animals were injected with tamoxifen to generate Nox4 ^−/− Spc-Cre animals and IHC was performed on the harvested lungs. (A) Representative immunofluorescent micrographs of lung tissue showing individual staining as well as co-staining of NOX4 and AT2 pneumocyte marker SPC (merge of green and red). Co-localization of NOX4 (green) and SPC (red) could be seen in lungs of Nox4 ^fl/fl mice, but not in Nox4 ^−/− Spc-Cre mice as indicated with white arrows (B) Quantified data of NOX4 fluorescence intensity from NOX4-SPC co-stained lung tissue (C) Expression of NOX4 in primary AT2 cells isolated from Nox4 ^fl/fl and Nox4 ^−/− Spc-Cre mice lung. n = 3 ****p < 0.0001.