FIGURE 8.

Silencing NOX4 reduces HO-induced increase in mt ROS production and apoptosis. (A) Representative confocal images of scRNA and NOX4 siRNA treated MLE-12 cells exposed to NO or HO and stained with MitoSOX (B) Quantitative data of mt ROS intensity measured as arbitrary units showed a decrease in HO-induced mt ROS with inhibition of NOX4 activity (C) Amplex red H₂O₂ assay of the cell culture supernatant showed increased release of H₂O₂ from MLE-12 cells exposed to HO compared to NOX4 siRNA treated cell supernatant (D) Immunoblots for NOX4 protein showed a significant reduction in the siRNA treated cells relative to control scRNA cells confirming efficient transfection. n = 3. ***p < 0.001, **p < 0.01, *p < 0.05.