FIGURE 9.

NOX4 expression regulates HO-induced apoptosis in lung epithelial cells. MLE-12 cells were transfected with NOX4 siRNA or control scrambled RNA for 24 h before HO exposure and apoptosis was assessed by flow cytometry. (A) Representative dot plots of cells stained with Annexin and 7-AAD (B) Graphs representing the percent fold-change of Annexin V- positive cells showing an increase in percentage fold change of apoptosis in HO group which was reduced in the siRNA treated cells. n = 3. ***p < 0.001, *p < 0.05.