TABLE 1.
Prepn | Total protein (mg) | Sp act (μmol/ min-mg) | Total activity (U)a | Yield (%) | Purification (fold) |
---|---|---|---|---|---|
Crude extract | 1,440 | 0.17 | 240 | 100 | 1 |
Heat treatment | 648 | 0.42 | 273 | 114 | 2.5 |
Q-Sepharose fast flow | 153 | 1.20 | 184 | 77 | 7.2 |
Phenyl-Sepharose | 48 | 3.47 | 163 | 68 | 21 |
Prep cell | 3 | 13.6 | 41 | 17 | 82 |
Gel filtration | 0.6 | 45.0 | 27 | 11 | 271 |
One unit was defined as 1 μmol of Pi produced per min at 95°C.