TABLE 1.

Partial purification of I-1-Pase from T. maritima

Prepn	Total protein (mg)	Sp act (μmol/ min-mg)	Total activity (U)a	Yield (%)	Purification (fold)
Crude extract	1,440	0.17	240	100	1
Heat treatment	648	0.42	273	114	2.5
Q-Sepharose fast flow	153	1.20	184	77	7.2
Phenyl-Sepharose	48	3.47	163	68	21
Prep cell	3	13.6	41	17	82
Gel filtration	0.6	45.0	27	11	271

^aOne unit was defined as 1 μmol of P_i produced per min at 95°C.