TABLE 3.
Substrate | Km (mM) |
Vmax (μmol of Pi min−1 mg of protein−1)
|
|
---|---|---|---|
Partially purified enzyme | Recombinant enzyme | ||
dl-I-1-P | 0.145 ± 0.016a | 21.3 ± 0.6a | |
d-I-1-P | 0.148 ± 0.013a | 44.6 ± 1.1a | |
0.126 ± 0.010b | 443 ± 10b | ||
l-I-1-P | 23c | ||
I-2-P | 0.460 ± 0.05a | 5.8 ± 0.3a |
The Km and Vmax values were determined with partially purified I-1-Pase (although the same relative specificities were observed with the recombinant enzyme).
Determined with homogeneous recombinant enzyme.
Estimated from the relative rates of the recombinant enzyme with pure d-I-1-P and with l-I-1-P.