TABLE 4.

Comparison of the kinetic characteristics of T. maritima I-1-Pase and I-1-Pases purified from other organisms

I-1-Pase source	Km (mM)	Vmax (μmol min−1 mg−1)	kcat/Km (M−1 s−1)	Assay temp (°C)	Reference
T. maritima	0.148 ± 0.013	∼450a	1.45 × 106	95
Recombinant T. maritima	0.126 ± 0.010	443 ± 10	1.67 × 106	95
Recombinant M. jannaschii	0.091 ± 0.016	9.3 ± 0.45	4.87 × 104	85	11
Bovine	0.16 ± 0.02	13.3 ± 0.9	3.96 × 104	37	19
Recombinant human	0.075 ± 0.003	36.1 ± 1	2.29 × 105	37	35
		8.1 ± 0.19	5.15 × 104	37	40
Recombinant E. coli	0.071 ± 0.008	13.3 ± 0.9	8.93 × 104	37	34

^aExtrapolated by using the V_max of the partially purified material and assuming that at most the 29-kDa band represented 10% of the total protein.