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. 2022 May 19;12:875146. doi: 10.3389/fonc.2022.875146

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Copyright © 2022 Chen, Chen, Pan, Zheng, Hong and Han

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Downregulation of KRT8 inhibited cell proliferation and induced cell apoptosis in HCC827 and H1975 cells. (A) Evaluation of KRT8 knockdown efficiency by Western blot in HCC827 and H1975 cells after transfection with KRT8 shRNA. (B) Downregulation of KRT8 inhibits cell proliferation of HCC827 and H1975 cells measured by CCK-8 assay. (C) Downregulation of KRT8 induces cell apoptosis of HCC827 and H1975 cells. Annexin V as an assay of apoptosis. (D) Downregulation of KRT8 inhibits cell proliferation of HCC827 and H1975 cells measured by colony formation assay (Student’s t-test, **P < 0.01 and ***P < 0.001).