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. 2022 May 18;25(6):104428. doi: 10.1016/j.isci.2022.104428

Figure 4.

Figure 4

Fusion of pVir-CR-HvKP5 and p3-CR-HvKP5 by IS26

(A) alignment of plasmid pCR-HvKP5TC-5_Vir-p3 with plasmid pVir-CR-HvKP5 and p3-CR-HvKP5 by easyfig, a colour gradient indicating the identities of the BLAST hits was present.

(B) alignment of plasmid pCR-HvKP5TC-5_Vir-p3 with plasmid pVir-CR-HvKP5 and p3-CR-HvKP5 by BRIG.

(C) Illustration of the process of fusion by pVir-CR-HvKP5 and p3-CR-HvKP5 by IS26. The structure of the insertion sequences was indicated. Left (IRL, GGCACTGTTGCAAA) and right (IRR, TTTGCAACAGTGCC) terminal 14 bp IRs were shown as yellow filled arrows. The 8 bp direct target repeats (CTTCGTAA) were shown as gray filled arrows.

(D) PCR assays to confirm the presence of the hybrid plasmid, pCR-HvKP5TC-5_Vir-p3 in CR-HvKP donor strains and their transconjugants.