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. 2022 May 18;25(6):104428. doi: 10.1016/j.isci.2022.104428

Figure 5.

Figure 5

Fusion of pVir-CR-HvKP3 and p3-CR-HvKP3 by IS903B

(A) alignment of plasmid pCR-HvKP3TC-2_Vir-p3 with plasmid pVir-CR-HvKP3 and p3-CR-HvKP3 by easyfig, a colour gradient indicating the identities of the BLAST hits was present.

(B) alignment of plasmid pCR-HvKP3TC-2_Vir-p3 with plasmid pVir-CR-HvKP3 and p3-CR-HvKP3 by BRIG.

(C) Illustration of the process of fusion by pVir-CR-HvKP3 and p3-CR-HvKP3 by IS903B.

(D) PCR assays to confirm the presence of the hybrid plasmid, pCR-HvKP3TC-2_Vir-p3 in donor strain CR-HvKP3 and transconjugants CR-HvKP3TC-2 and CR-HvKP3TC-4.