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. 2022 Jan 31;11(11):2244–2258. doi: 10.1002/cam4.4547

FIGURE 6.

FIGURE 6

Effects of CAPN8, IRX2, and SPINK13 on cell death in A549 and NCI‐H1299 cell lines. (A) The three genes' expression levels by qRT‐PCR after silencing with mixed siRNAs in A549 transfected with GFP (A549‐GFP) cells for 48 h. Data are shown as the mean ± SD from two biological replicates (n = 2). (B) The three genes' expression levels by qRT‐PCR after silencing with mixed siRNAs in NCI‐H1299 transfected with GFP (H1299‐GFP) cells for 48 h. Data are shown as the mean ± SD from two biological replicates (n = 2). (C) Percentages of annexin V+ populations after gene silencing in A549 cell lines. Left: Representative original histogram overlays of fluorescence reflecting apoptosis. Top right: Quantification of annexin V+ populations are shown as the mean ± SD from three biological replicates (n = 3). (D) Percentages of annexin V+ populations after gene silencing in NCI‐H1299 cell lines. Left: Representative original histogram overlays of fluorescence reflecting apoptosis. Top right: Quantification of annexin V+ populations are shown as the mean ± SD from three biological replicates (n = 3). *p < 0.05; **p < 0.01; ns: Nonsignificance (Student's t‐test)