TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Reference |
|---|---|---|
| Bacterial strains | ||
| E. coli HB101 | recA mutant, used for transformation of RSF1010 derivatives | 6 |
| A. radiobacter AD1 | Utilizes dihalogenated propanols | 34 |
| Pseudomonas sp. strain GJ1 | Utilizes 2-chloroethanol | 14 |
| P. putida US2 | Utilizes 2-chloroethanol | 33 |
| P. putida GJ31 | Utilizes long-chain alcohols | 25 |
| Rhodococcus sp. strain m15-3 | DhaA-producing strain | 39 |
| Plasmids | ||
| pKK233-2 | Apr, Ptrc | 1 |
| pGEF+ | Apr, PT7 | 28 |
| pKKdhaA | dhaA gene cloned into the NcoI site of pKK233-2 | This work |
| pDSK519 | RSF1010 derivative, Kmr, Plac | 19 |
| pJRD215 | RSF1010 derivative, Kmr | 9 |
| pRK600 | Cmr, transfer functions for mobilization of RSF1010 derivatives | 11 |
| pTB1 | dhaA inserted as a 0.9-kb BamHI fragment in pDSK519 | This work |
| pTB3 | 1.5-kb HindIII-BamHI PCR fusion fragment of PdhlA-dhaA inserted in pJRD215 | This work |
| pTB5 | 1.4-kb BamHI fragment of Ptrc-dhaA-rrnB transcription terminator inserted in pJRD215 | This work |
a
Ap, ampicillin; Km, kanamycin; Cm, chloramphenicol.