TABLE 2.
Steady-state parameters and products of trihalogenated propane conversion of haloalkane dehalogenase
| Substrate | DhaA
|
DhlA
|
Halogenated product formed | ||||
|---|---|---|---|---|---|---|---|
| kcat (s−1) | Km (mM) | kcat/Km (s−1 M−1) | kcat (s−1) | Km (mM) | kcat/Km (s−1 M−1) | ||
| 1,2,3-Tribromopropane | 3.6 | 0.3 | 1.2 × 104 | >0.9 | >2.5b | 360 | 2,3-Dibromo-1-propanol |
| 1,2-Dibromo-3-chloropropane | 2.3 | 1.0 | 2,300 | >0.7 | >3.0b | 233 | 2-Bromo-3-chloro-1-propanol |
| 1,2,3-Trichloropropane | 0.08 | 2.2 | 36 | >0.01 | >10b | 1 | 2,3-Dichloro-1-propanol |
| 1,2-Dibromoethane | 14.3 | 3.9 | 3,660 | 3.0a | 0.01a | 3.0 × 105 | 2-Bromoethanol |
a
See reference 32.
b
Highest concentration checked. The Km values had errors ≤25%, and the kcat values had errors ≤15%.