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. 2022 May 19;13:861105. doi: 10.3389/fphar.2022.861105

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Copyright © 2022 Han, Yuan, Chen, Huang, Wang, Zhou, Dong, Zhang, Zhang, Yin and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Verification and screening of anti-inflammatory activity of active components in Raw264.7 cells. (A) Viability of Raw264.7 cells. Cells were treated with 50 μM corresponding reagents and then measured by MTT assay, following by the normalization of cell viability. (B) Screening results in Raw264.7 cells. Cells were treated with 1 µg/ml LPS and 50 μM corresponding reagents, and then measured the release of NO, followed by the normalization of inhibition rate. (C) Dose-dependent inhibitory response of LPS-induced NO release in Raw264.7 cells. Cells were treated with 1 µg/ml LPS and serial concentration of chrysin and oroxylin A (0.78, 1.56, 3.12, 6.25, 12.5, 25, and 50 μM) and then measured the release of NO, followed by the normalization of inhibition rate. All data were reported as the mean of three independent experiments.