FIGURE 7.

Oroxylin A suppressed the activation of TLR4/NF-κB signaling pathways in vitro and in vivo. (A) Related proteins of the TLR4-NFκB pathway evaluated by Western blot assay. (B) Statistical results of the protein levels of MyD88, IRAK4, p-TAK1, p-IKKβ, p-IκBα, and p-p65. The band intensity was normalized by β-actin and calculated according to the gray level. (C) Activities of oroxylin A (50 µM) in HEK-Blue hTLR4 cells and HEK-Blue null cells evaluated by measuring the expression of SEAP signal-induced by LPS (1 µg/ml). (D) Cell viability of HEK-Blue hTLR4 cells and HEK-Blue null cells with different treatment of LPS (1 µg/ml) or oroxylin A (50 µM) by MTT assay. The samples were n = 3 per group. Data are presented as mean ± SD. #p < 0.05 vs. control, ##p < 0.01 vs. control, *p < 0.05 vs. model, **p < 0.01 vs. model.