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. 2022 May 19;16:876582. doi: 10.3389/fnins.2022.876582

FIGURE 1.

FIGURE 1

Gavage of periodontitis salivary microbiota aggravates ischemic stroke in mice. (A) Experimental design for testing the effects of saliva of periodontitis patients. AMNV, ampicillin, metronidazole, neomycin sulfate, and vancomycin. (B) Representative TTC-stained images of mouse cerebral sections 1 day after stroke. TTC, 2, 3, 5-triphenyl-2H-tetrazolium chloride. MCAO, middle cerebral artery occlusion. ASDW, antibiotics + sterile distilled water + MCAO. ASPP, antibiotics + saliva of periodontitis patients + MCAO. (C) Quantification of infarct volume. n = 8:8. (D) Neurological scores of mice 1 day after stroke. n = 8:8:8:8. (E) qPCR analysis of 16s rRNA gene copies in 1ul saliva and its filtrate. AFSP, antibiotics + filtrate of the saliva of periodontitis patients + MCAO. n = 6:7. (F) Representative TTC-stained images of mouse cerebral sections 1 day after stroke. (G) Quantification of infarct volume. n = 5:5. Values represent mean ± SD. One-Way ANOVA was used for statistical analysis in (C,D), and Student’s t-test was used in (G). *p < 0.05, ***p < 0.001, ****p < 0.0001.