FIGURE 2.

Gavage of periodontitis salivary microbiota promotes inflammation in the ischemic region. (A) Left, representative immunofluorescence staining of Iba-1 in the ischemic region of the brain. Scale bar = 100 μm. Right, quantification of Iba-1-positive cells. n = 5:5:5:5. (B) Left, representative immunofluorescence staining of GFAP in the ischemic region of the brain. Scale bar = 100 μm. Right, quantification of GFAP-positive cells. n = 5:5:5:5. (C) qPCR analysis of relative mRNA expression of Il-1β and Tnf-α in the ischemic brain. n = 7:12:8:10. (D) IL-1β and TNF-α detected by ELISA in the ischemic brain. n = 4:4:4:4. (E) Representative flow cytometry analysis of CD45^highCD11b^– cells and CD45^highCD11b⁺ cells in ischemic brain. (F) Quantification of CD45^highCD11b^– cells. n = 6:6:6:6. (G) Quantification of CD45^highCD11b⁺ cells. n = 6:6:6:6. (H) Representative flow cytometry analysis of CD11b⁺Ly6G⁺ cells. (I) Quantification of CD11b⁺Ly6G⁺ cells. n = 5:5:5:5. (J) Representative flow cytometry analysis of CD86 and CD206 in CD45^highCD11b⁺ cells. (K) Quantification of CD45^highCD11b⁺CD86⁺ cells. n = 6:6:6:6. Values represent mean ± SD. One-Way ANOVA was used for statistical analysis. *p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001.