FIGURE 4.

Gavage of periodontitis salivary microbiota increases Th17 and IL-17⁺ γδ T cells in the small intestine. (A) Left, representative flow cytometry analysis of CD4⁺IL-17A⁺ cells (Th17 cells) in the small intestine. Right, quantification of Th17 cells. n = 5:5:6:6. (B) Left, representative flow cytometry analysis of TCR-γδ⁺IL-17A⁺ cells (IL-17⁺ γδ T cells) in the small intestine. Right, quantification of IL-17⁺ γδ T cells. n = 5:5:5:5. (C) Left, representative flow cytometry analysis of CD4⁺RORγt⁺ T cells in the small intestine. Right, quantification of CD4⁺RORγt⁺ cells. n = 6:6:6:6. (D) Left, representative flow cytometry analysis of TCR-γδ⁺RORγt⁺ cells in the small intestine. Right, quantification TCR-γδ⁺RORγt⁺ cells. n = 5:5:5:5. Values represent mean ± SD. One-Way ANOVA was used for statistical analysis. *p < 0.05, ^***p < 0.001, ^****p < 0.0001.