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. 2022 May 19;16:876582. doi: 10.3389/fnins.2022.876582

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Copyright © 2022 Chen, Bai, Dilimulati, Shao, Qiu, Liu, Xu, Bai, Du, Zhou, Lin, Meng, Jin, Liu, Zhang, Duan and Jia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intestinal immune cells rapidly respond to ischemic stroke. (A) Representative flow cytometry analysis of immune cells in the small intestine 1d after MCAO or sham operation. (B) Quantifications of immune cells in the small intestine. n = 5:5. (C) Strategy for analyzing intestinal immune cell trafficking to the brain by using Kaede transgenic mice. (D) Representative flow cytometry analysis of photoconverted CD4⁺ T cells and γδ T cells in the ischemic brain. (E) Quantifications of photoconverted CD4⁺ T cells and γδ T cells. n = 4:6. Values represent mean ± SD. Student’s t-test was used for statistical analysis. ns, not significant; *p < 0.05 ^**p < 0.01, ^***p < 0.001.