Extended Data Fig. 3 |. Identification of synthetically lethal genes in TRF2-deficient ES cells.

a, Scatter plots from three independent CRISPR–Cas9 screens from Fig. 2b. Genes that are synthetically lethal with TRF2 knockout are labelled with dark blue dots. Genes that are essential for ES cells are labelled with magenta dots. b, Heat map displaying the essentiality score (β-score) of the 13 candidate synthetically lethal genes (dark blue), and for 8 genes that have previously been described as essential in ES cells³¹ (purple), across 3 independent experiments. c, Schematic of the Pot1b and Brd2 genomic loci showing genome organization, the predicted CRISPR–Cas9 cut site (scissors, red) and key domains required for protein function (blue). Sanger sequencing of Pot1b^−/− (exon 2) and Brd2^−/− (exon 4) for different clones isolated is shown. For Pot1b, a repair template cassette containing multiple STOP codons was used. gRNA sequences are highlighted in red.