TABLE 3.
Ammonia oxidation by butane-grown bacteria
| Addition | Oxidation products (nmol)a
|
|||||
|---|---|---|---|---|---|---|
|
P. butanovora
|
CF8
|
M. vaccae
|
||||
| NH2OH | NO2− | NH2OH | NO2− | NH2OH | NO2− | |
| None | 94 ± 24 | 1.4 ± 0.5 | 13.1 ± 2.9 | 10.5 ± 1.3 | <1 | 18.1 ± 3.2 |
| Butyrateb | 226 ± 24 | 8.0 ± 0.2 | 5.7 ± 2.8 | 15.0 ± 2.1 | <1 | 4.8 ± 1.0 |
| Acetylene (10%)c | <1 | <1 | <1 | 3.9 ± 1.0 | <1 | <1 |
| Butane (10%) | <1 | <1 | <1 | 3.8 ± 1.0 | <1 | <1 |
a
Each reaction mixture contained NH4Cl (10 mM) and the indicated addition. Assays were conducted for 3 h for CF8 and M. vaccae and for 2 h for P. butanovora (approximately 0.17, 0.36, and 0.15 mg of protein, respectively). Data are expressed as means ± standard deviations.
b
5 mM sodium butyrate for M. vaccae and 0.5 mM for CF8 and P. butanovora.
c
Vol/total vial vol.