Table 2.
List of up-regulated and down-regulated sperm proteins in male hypogonadism (n=32 proteins down-regulated; n=11 proteins up-regulated).
| Accession | Gene name | Description | Peptide count | Unique Peptides | Ratio Hypo/Ctl |
|---|---|---|---|---|---|
| Down-regulated proteins in male hypogonadism sperm samples (n=32) | |||||
| Q9Y272 | RASD1 | Dexamethasone-induced Ras-related protein 1 | 1 | 1 | 0.425 |
| P12273 | PIP | Prolactin-inducible protein | 3 | 3 | 0.514 |
| P05164 | MPO | Myeloperoxidase | 1 | 1 | 0.532 |
| P02768 | ALB | Serum albumin | 15 | 11 | 0.579 |
| P05109 | S100A8 | Protein S100-A8 | 1 | 1 | 0.597 |
| Q08380 | LGALS3BP | Galectin-3-binding protein | 1 | 1 | 0.604 |
| Q9UBC9 | SPRR3 | Small proline-rich protein 3 | 1 | 1 | 0.632 |
| P05154 | SERPINA5 | Plasma serine protease inhibitor | 1 | 1 | 0.633 |
| O14607 | UTY | Histone demethylase UTY | 1 | 1 | 0.640 |
| Q02383 | SEMG2 | Semenogelin-2 | 22 | 18 | 0.644 |
| P08118 | MSMB | Beta-microseminoprotein | 1 | 1 | 0.647 |
| P07288 | KLK3 | Prostate-specific antigen | 1 | 1 | 0.647 |
| P25311 | AZGP1 | Zinc-alpha-2-glycoprotein | 2 | 2 | 0.656 |
| Q15843 | NEDD8 | NEDD8 | 1 | 1 | 0.663 |
| P04279 | SEMG1 | Semenogelin-1 | 18 | 14 | 0.664 |
| Q8TCT9 | HM13 | Minor histocompatibility antigen H13 | 1 | 1 | 0.666 |
| Q99963 | SH3GL3 | Endophilin-A3 | 1 | 1 | 0.688 |
| O15127 | SCAMP2 | Secretory carrier-associated membrane protein 2 | 1 | 1 | 0.702 |
| Q6W4X9 | MUC6 | Mucin-6 | 1 | 1 | 0.705 |
| Q9Y5C1 | ANGPTL3 | Angiopoietin-related protein 3 | 1 | 1 | 0.705 |
| Q4G0P3 | HYDIN | Hydrocephalus-inducing protein homolog | 1 | 1 | 0.709 |
| Q5JQC9 | AKAP4 | A-kinase anchor protein 4 | 34 | 33 | 0.710 |
| P07602 | PSAP | Prosaposin | 2 | 2 | 0.711 |
| A4D1T9 | PRSS37 | Probable inactive serine protease 37 | 2 | 2 | 0.724 |
| Q4W5G0 | TIGD2 | Tigger transposable element-derived protein 2 | 1 | 1 | 0.733 |
| Q9UIA9 | XPO7 | Exportin-7 | 2 | 2 | 0.733 |
| O75969 | AKAP3 | A-kinase anchor protein 3 | 25 | 25 | 0.742 |
| Q9H4F8 | SMOC1 | SPARC-related modular calcium-binding protein 1 | 1 | 1 | 0.745 |
| Q0VFZ6 | CCDC173 | Coiled-coil domain-containing protein 173 | 1 | 1 | 0.750 |
| Q9BTW9 | TBCD | Tubulin-specific chaperone D | 1 | 1 | 0.750 |
| Q9NZL4 | HSPBP1 | Hsp70-binding protein 1 | 1 | 1 | 0.759 |
| Q9BZX4 | ROPN1B | Ropporin-1B | 6 | 1 | 0.768 |
| Up-regulated proteins in male hypogonadism sperm samples (n=11) | |||||
| P02751 | FN1 | Fibronectin | 13 | 13 | 1.272 |
| Q7Z5L4 | SPATA19 | Spermatogenesis-associated protein 19, mitochondrial | 1 | 1 | 1.280 |
| Q12765 | SCRN1 | Secernin-1 | 1 | 1 | 1.283 |
| O14841 | OPLAH | 5-oxoprolinase | 1 | 1 | 1.289 |
| P62750 | RPL23A | 60S ribosomal protein L23a | 1 | 1 | 1.296 |
| Q9NS25 | SPANXB1 | Sperm protein associated with the nucleus on the X chromosome B1 | 4 | 3 | 1.386 |
| P49901 | SMCP | Sperm mitochondrial-associated cysteine-rich protein | 2 | 2 | 1.387 |
| Q96BH3 | ELSPBP1 | Epididymal sperm-binding protein 1 | 2 | 2 | 1.468 |
| Q2KHT4 | GSG1 | Germ cell-specific gene 1 protein | 1 | 1 | 1.656 |
| Q17RY6 | LY6K | Lymphocyte antigen 6K | 1 | 1 | 1.672 |
| Q9H3G5 | CPVL | Probable serine carboxypeptidase CPVL | 1 | 1 | 1.987 |
The criteria used to accept protein identification included at least 1 unique peptide and an FDR of 1%. Results are expressed as the protein ratio of sperm proteins from secondary hypogonadism patients (Hypo) to controls (Ctl).