(a-c) Schema of experimental approach. (d-g) Left panels: average calcium traces (ΔF/F) for indicated response types. Shaded area indicates s.e.m. Right panels: response probability expressed as fraction of stimuli leading to a response. P = 6.4 × 10−3 for ON, P = 4.31 × 10−2 for Sustained, and P = 3.21 × 10−1 for OFF. Bar graph plotted as mean ± s.e.m. *P < 0.05, **P < 0.01, ****P < 0.0001, two-sided Mann-Whitney test. (h) 150 randomly chosen Z-scored example traces showing activity during six consecutive stimuli (shaded) and corresponding ITIs. (i) Cumulative probability distribution of Ca2+ transient number and amplitude during intertrial interval (ITI). ****P < 0.0001, Kolmogorov-Smirnov test (n = 580 neurons for WT and n = 277 neurons for SRGAP2C).