Extended Data Figure 4. Connectivity changes are not caused by differences in cortical depth or number of starter neurons.

(a) Anatomical location of starter neurons. (b) Cortical depth of starter neurons measured as distance from pial surface is not different between WT and SRGAP2C mice, two-sided Mann-Whitney test. Data shown as box-and-whisker plots. Center line indicates median, box edges represent first and third quartiles, and whiskers represent minimum and maximum values (n = 26 starter neurons from 10 WT mice and n = 26 starter neurons from 7 SRGAP2C mice). (c) Correlation between number of RABV infected starter neurons and RABV traced neurons (Pearson’s correlation coefficient r = 0.88, P = 7 × 10⁻⁴ for WT, and r = 0.92, P = 3.2 × 10⁻³ for SRGAP2C). (d) No correlation was observed between IOC and number of RABV infected starter neurons per brain (Pearson’s correlation coefficient r = −0.5, P = 0.14 for WT, and r = −0.58, P = 0.17 for SRGAP2C).