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Binding epitope mapping of the G0-peptide with FUT8 by STD NMR in (a) the absence and (b) presence of GDP. Protein saturation was achieved by irradiation at −0.50 ppm. The colored circles represent the normalized STD-NMR intensity. Only STD responses are indicated for protons that could be accurately measured. Hollow circles indicate the sum of STD intensities of overlapping GlcNAcE and GlcNAcG.
