Table 1. Kinetic Parameters for the FUT8 Glycosylation of the Different N-glycan and Asn-Linked Oligosaccharides and N-glycopeptides Used in This Study Using FUT8d.
| Km (μM) | Vmax (nmol·min–1·mg–1) | kcat (min–1) | kcat/Km (min–1·μM–1) | |
|---|---|---|---|---|
| GDP-Fuca | 14.56 ± 3.4 | 244.3 ± 13.1 | 14.17 ± 0.76 | 0.97 |
| G0 | 113.1 ± 15.43 | 282.1 ± 11.72 | 15.62 ± 0.5 | 0.14 |
| G0-peptide | 133.1 ± 19.99 | 224.7 ± 10.68 | 13.03 ± 0.62 | 0.1 |
| M3N2 | b | b | b | b |
| M3N2-peptide | c | c | c | c |
| M5N2-Asn | c | c | c | c |
a
The Km of GDP-Fuc was determined in the presence of a saturating concentration of G0.
b
Not active.
c
Kinetic parameters not determined (data could not be fitted to the nonlinear Michaelis–Menten equation because, under our conditions, FUT8 showed a linear increase in activity versus concentration of the N-glycans).
d
Note that the first row defines the kinetic parameters for the donor substrate GDP-Fuc.